Talking glycoproteomics with Wells and Hart
Lance Wells and Gerald “Jerry” Hart are certified glycobiology geeks.
Hart got his start working with proteoglycans, proteins that are heavily decorated with glycans, and has spent the last 37 years studying O-GlcNAcylation, a nutrient- and stress-responsive post-translational modification. Since 2018, he’s been a professor and Georgia Research Alliance eminent scholar at the University of Georgia.
Wells joined Hart’s lab as a postdoc and became an expert in mass spectrometry. He is now a professor and Georgia Research Alliance distinguished investigator at the University of Georgia Complex Carbohydrate Research Center, or CCRC.
91影库Today talked to Wells and Hart about glycobiology and their in Molecular & Cellular Proteomics. The interview has been edited.
How did you get interested in glycobiology?
GH: Glycobiology is one of the last frontiers in biochemistry, partly due to the complexity and diversity of the molecules. Glycosylation is the most abundant post-translational modification by far. I founded the journal Glycobiology in 1989 and served as editor-in-chief for 12 years. So I've been involved with every aspect of the field for a long time. Mass spectrometry is probably the most important essential tool in analyzing post-translational modifications, particularly glycosylation. I’ve been involved with proteomics as well, or glycomics, as we call it.
LW: I went to Emory University School of Medicine for my Ph.D., for one reason — to work with Keith Wilkinson, who is an expert in ubiquitin biology. But first we had to do rotations. So I went to Judy Fridovich–Keil’s lab, and I just fell in love with the power of genetics. In that lab, we studied galactosemia, the synthesis of sugar nucleotides and how defects can cause congenital disease. I ended up staying in Judy’s lab, and when it came time to do a postdoc, I wanted to take the next step by asking, “What are these sugar nucleotides actually used for?” It hadn’t been that long since Jerry Hart’s lab discovered the O-GlcNAc modification, which seemed like a really cool way for cells to take into account their nutrient status. Jerry took me into his lab, and I stayed for five years and developed a lot of mass spectrometry skills. Then I joined the CCRC, where I’ve been studying O-GlcNAc and other types of glycosylation. I started collaborating with Kevin Campbell, who’s a Howard Hughes investigator who studies O-mannosylation. (Note: Campbell is also this year’s Herb Tabor Award winner.) Now half my lab does O-mannosylation and half does O-GlcNAc. That’s how I got into it and just kind of fell in love.
How has mass spec evolved in glycoproteomics?
GH: Lance is probably one of the top glyco mass spec people on the planet. He learned his mass spectrometry skills on his own when he was in my lab, where we did liquid chromatography–mass spectrometry, which is very primitive compared to what they have now. Lance basically pioneered the use of mass spec in our lab.
LW: We were on a Paul trap in Jerry’s lab, which at the time was a workhorse, just an amazing instrument. But the amount of data I generated in all my years there could probably be generated in a week today. Certain key technologies have catalyzed progress. We used to do everything by collision-induced dissociation, or CID, which was our only option to dissociate molecules for tandem mass spectrometry. Now we have so many electron dissociation techniques, but what probably doesn’t get enough attention from biologists is the backend software. In this special MCP issue, about a third of the articles are about how to turn the data that’s generated into something we can use in biology.
GH: Probably the most astonishing thing about the development of mass spectrometry in the last decade is the increase in sensitivity and mass accuracy. The very first MALDI (matrix-assisted laser desorption/ionization) instrument I owned was about as accurate as gel filtration. They have gotten much better. The development of the orbitrap was a major breakthrough.
topic areas, from top to bottom. The first is capturing glycoproteins/glycopeptides
(purple background) from the nonglycosylated species (green). The purple arrow
represents introduction into a mass spectrometer for analyses by fragmentation
(yellow). Finally, the area of post-acquisition data analyses (blue) takes the data
generated(yellow arrow) and reassembles the pieces to map sites of modification
and determine site-specific microheterogeneity (mosslike structures) on the protein
backbone(stones at bottom).
Why an MCP glycoproteomics special issue now?
LW: Eight years ago, we put together a special issue about glycomics — the characterization of glycans released from proteins. Since then, the field has moved forward fast. Hardware, sensitivity, separations and tools to analyze data have made tremendous progress. So, in this special issue, about one-third of the articles are about enriching for glycoproteins or glycopeptides, one-third are about analyzing them on a mass spectrometer, and one-third are, OK, you’v